The work aims to explore the design theory and method of mass entrepreneurship and innovation space based on campus culture and regional culture, enrich the design theory system of mass entrepreneurship and innovation block, and better guide the spatial design practice of national mass entrepreneurship and innovation demonstration base with innovation and entrepreneurship as the core. Starting with the concept of school-land integration and mass entrepreneurship and innovation block, Nanjing“Zijin Creative Valley”(a mass entrepreneurship and innovation block) is taken as the research object. The work expounds the advantages and main contradictions of the“mass entrepreneurship and innovation”base through field research and comparative analysis to probe into the space design strategy of Nanjing“Zijin Creative Valley”based on the school-land integration. The mass entrepreneurship and innovation cultural space model based on school-land integration is the practice of the construction of the national innovation and entrepreneurship system, which can not only re-stimulate the vitality of colleges and universities and surrounding areas to achieve their sound development and form a strong innovation resource cluster, but also enhance and improve their own and the overall innovationability, achieving win-win economic and social benefits.