The cultural genes of the anti-dyeing technology of the ethnic minorities in South Guizhou are not only an important part of the cultural genes of ethnic minorities in South Guizhou, but also the "key" to interpret the cultural codes of this technology groups. Based on existing classification methods on folk craft culture genes, this paper explores the morphological characteristics of the culture genes of the anti-dyeing technology of the ethnic minorities in South Guizhou, and analyzes the formation factors from multiple angles. In the diachronic development process, the cultural genes of the anti-dyeing technology of the ethnic minorities in South Guizhou are affected by various factors such as natural conditions, primitive religious beliefs and customs of this technology groups, and the multi-ethnic culture blending, etc., which are conducive to fully reveal their special material culture and spiritual culture.