In order to improve user satisfaction and operation efficiency of the mobile medical interactive interface for the elderly, a user-centered development method for mobile medical interactive interface for the elderly was proposed to optimize user experience. Firstly, the main design elements of the mobile medical interactive interface for the elderly were analyzed. The interactive interface design scheme required for experiment was determined through an orthogonal experiment. Factor analysis was used to clarify the key items of the user evaluation system and structure the evaluation hierarchy. Secondly, the conjoint analysis method was used to obtain the design elements of the mobile medical interactive interface and the utility value of its type. According to the experimental results, the preference of elderly users on the interactive interface of mobile medical APPs was obtained, and the optimal interface design elements were discovered, so as to formulate corresponding design strategies. Finally, according to the demands and preferences of the elderly, the user experience of medical APPs for the elderly was effectively optimized. Furthermore, the interface interaction prototype was designed, so as to provide references for designers to develop APPs that meet the needs of the elderly.