The work aims to study the visualization design strategy of amblyopia medical knowledge to promote the effective dissemination of amblyopia medical knowledge, and explore the construction of a visualization design application system model to provide a new paradigm for the visualization design of amblyopia medical knowledge. Starting from user needs, the influencing factors of users' information receiving and recalling are analyzed to obtain users' needs for amblyopia knowledge through questionnaires and interviews, construct a hierarchical analysis system of user needs from five levels of sensation, function, knowledge, psychology and interaction by hierarchical analysis, quantify the evaluation indexes of each factor under each level, compute the weights of each factor at each level, and then analyze the user needs and preferences. A basic strategy of visualization design for amblyopia medical knowledge is proposed and applied in practice. After extracting the important user need factors, the design direction is summarized to explore the visualization method of amblyopic medical knowledge, and verify the feasibility of the medical knowledge visualization module by practical application, so as to provide reference for the visualization and effective dissemination of amblyopic medical knowledge.