In the context of new materialism, the study discusses the ongoing episteme-shift in recent design practices and research of fungal biodesign, and analyzes the causes and characters of this trend, so as to shed light on ways to enable effective interdisciplinary cooperation and cope with global challenges in the crisis of Anthropocene. This paper examines the history of fungal biodesign. By comparing the discovery of modern science about fungi, the ways to obscure its aliveness, and the rediscovery of such aliveness through the lens of new materialism, the limitations of anthropocentrism are revealed. Through case analysis and introduction of the concepts of "assemblage" and "contamination" proposed by Anna Tsing in her study on fungi, the episteme inspired by fungi is summarized. The contribution of fungal biodesign lies not only in the update of design materials, but also the new episteme inspired by the aliveness of fungi, which has an impact on design ontologically, epistemologically and methodologically.